Methods of Protein Delivery into Mammalian Cells for Gene Therapy and Genetic Studies

Methods, lying on different principles for introducing proteins into mammalian cells were compared in the present study. Some of them rely on non-covalent complex formation (Chariot), osmotic lysisis of pinocytic vesicles (Influx pinocytic cell-loading reagent), electric power (electroporation), lipid-based delivery system (Bioporter), microinjection, small protein transduction domains (PTDs) from viral proteins, bacterial secretion system type III (T3SS). Thus, the delivery by many of the compared methods can give a sufficient number of uniformly loaded cells for different studies including transcription and translation, cell cycle regulation, control of apoptosis, oncogenesis and gene therapy.